Flow rate-controlled pipetting for microfluidics: second-generation flexible hydraulic reservoir (FHRv2)

A critical component of microfluidic technology is the fluid pumping mechanism. Syringe and pressure pumps are typically used in lab environment; however, their operations generate considerable dead volume that often larger than chip itself, leading to waste precious sample. As an alternative, pipetting allows for precise liquid dispensing with zero volume; it has a limited flow control. Recently, we have introduced low-cost sample loading interface dead-volume named flexible hydraulic reservoir (FHR). In this study, present second-generation FHRv2 combines continuous pumping, zero-dead versatility pipetting. The performance tested against syringe pump at rates ranging between 20 60 $${\upmu }$$ L/min. It demonstrated smoother operation identical transient time reach steady rate as confirmed by mathematical model developed occasion. Importantly, also demonstrate prevents sedimentation-induced artifacts encountered typical when particles. Finally, fabrication concept injection molding using 3D-printed mold. Overall, our offers versatile solution zero-volume handling devices.